实验动物科学 ›› 2023, Vol. 40 ›› Issue (1): 72-74.DOI: 10. 3969 / j. issn. 1006-6179. 2023. 01. 014

• 技术 • 上一篇    下一篇

改良小鼠腹腔巨噬细胞提取方法

  

  1. (沈阳医学院公共卫生学院,沈阳 110034)
  • 收稿日期:2021-05-19 出版日期:2023-02-28 发布日期:2023-03-15
  • 作者简介:喻道军( 1969—) ,男,博士,副教授,研究方向:环境因素对健康的影响. E-mail:174924428@ qq. com
  • 基金资助:
    辽宁省教育厅科学技术研究项目( L2012377)

Improved Extraction Method of Mouse Peritoneal Macrophage

  1. ( School of Public Health, Shenyang Medical College, Shenyang 110034, China)
  • Received:2021-05-19 Online:2023-02-28 Published:2023-03-15

摘要: 目的 改进小鼠腹腔巨噬细胞提取方法。 方法 从胸壁外侧肌肉进针,突破横膈膜进入腹腔,注入培养液,充分按揉后提取腹腔巨噬细胞。 结果 腹膜完整性得以保持,便于操作。 结论 优化了传统提取方式,保持腹腔完整性,使提取操作简便、高效、不易污染,尤其操作感良好,巨噬细胞提取数量有保证。

关键词: 腹腔巨噬细胞, 小鼠

Abstract: Objective To improve the extraction method of mouse peritoneal macrophage. Method Nutrient solution was injected into mouse enterocoelia across the lateral muscle of the chest wall and diaphragm, and the macrophages were extracted from the abdominal cavity after sufficient rubbing. Result Peritoneal integrity was maintained for ease of operation. Conclusion The traditional extraction method was optimized to maintain the integrity of the abdominal cavity, so that the extraction operation was simple, efficient and not easy to pollute, especially the operation feeling was good, and the number of be swayed by considerations of gain and loss and macrophage extraction was guaranteed.

Key words: peritoneal macrophage, mouse

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